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Measurement of interleukin 1 in pulmonary reactions induced by agricultural dusts.

Authors
Lewis-DM; Stasny-A; Bledsoe-TA
Source
Scand J Work, Environ & Health 1992 Jun; 18(Suppl 2):72-74
NIOSHTIC No.
00209449
Abstract
The interleukin-1 (IL-1) responses of U937 cells and peripheral blood monocytes to organic dusts were compared. Blood was collected from healthy volunteers. The analysis of IL-1beta in blood was accomplished by spiking blood samples, plasma, or serum with a known quantity of IL-1beta, allowing the blood or plasma to clot, and then analyzing the IL-1 content of the samples. Human peripheral blood mononuclear cells were used in the evaluation of IL-1beta production. The analysis of the IL-1beta content of blood and plasma revealed that the assay was not reliable for this purpose. The results of the study indicate that while IL-1 can be measured in serum and plasma, the values may be artificially elevated. This finding implies that circulating levels of IL-1 may not be a biomarker of pulmonary inflammation that can be used in field investigations. It may be useful in exposure studies where the change in IL-1 levels, rather than the absolute value, may be meaningful. Extracts of bulk cotton samples were tested with U937 cells and monocytes. The histiocytic cell line U937 was found to produce IL-1 in response to environmental samples and it may be useful in the evaluation of the inflammatory potential of such samples.
Keywords
NIOSH-Author; Analytical-methods; Chemical-analysis; Blood-analysis; Cotton-dust; In-vitro-studies; Blood-serum; Blood-cells
Contact
Dr DM Lewis, Immunology Section, NIOSH, 944 Chestnut Ridge Road, MS215, Morgantown, WV26505, USA
CODEN
SWEHDO
Publication Date
19920601
Document Type
Journal Article
Fiscal Year
1992
NTIS Accession No.
NTIS Price
ISSN
0355-3140
NIOSH Division
DRDS
Source Name
Scandinavian Journal of Work, Environment and Health
State
WV
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