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Measurement of interleukin 1 in pulmonary reactions induced by agricultural dusts.

Lewis-DM; Stasny-A; Bledsoe-TA
Scand J Work, Environ & Health 1992 Jun; 18(Suppl 2):72-74
The interleukin-1 (IL-1) responses of U937 cells and peripheral blood monocytes to organic dusts were compared. Blood was collected from healthy volunteers. The analysis of IL-1beta in blood was accomplished by spiking blood samples, plasma, or serum with a known quantity of IL-1beta, allowing the blood or plasma to clot, and then analyzing the IL-1 content of the samples. Human peripheral blood mononuclear cells were used in the evaluation of IL-1beta production. The analysis of the IL-1beta content of blood and plasma revealed that the assay was not reliable for this purpose. The results of the study indicate that while IL-1 can be measured in serum and plasma, the values may be artificially elevated. This finding implies that circulating levels of IL-1 may not be a biomarker of pulmonary inflammation that can be used in field investigations. It may be useful in exposure studies where the change in IL-1 levels, rather than the absolute value, may be meaningful. Extracts of bulk cotton samples were tested with U937 cells and monocytes. The histiocytic cell line U937 was found to produce IL-1 in response to environmental samples and it may be useful in the evaluation of the inflammatory potential of such samples.
NIOSH-Author; Analytical-methods; Chemical-analysis; Blood-analysis; Cotton-dust; In-vitro-studies; Blood-serum; Blood-cells
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Scandinavian Journal of Work, Environment and Health