Inhibition of intercellular communication in human keratinocytes by fractionated asphalt fume condensates.
Wey-HE; Breitenstein-MJ; Toraason-MA
Carcinogenesis 1992 Jun; 13(6):1047-1050
The effect of asphalt fume condensate fractions on gap junctional intercellular communication in a human epidermal keratinocyte cell line was investigated. Cultures of epidermal keratinocytes were exposed to medium containing one of five asphalt fume condensate fractions. Gap junction intercellular communication was assessed by the dye coupling method using fluorescent dye microinjection. The total number of dye positive adjacent cells were used as an indication of gap junction intercellular communication. Asphalt fume condensate fractions induced toxic effects that were dependent on the total mass of the added fraction. All the fractions demonstrated a concentration dependent inhibition of dye coupling, indicating an inhibition in gap junction intercellular communication. Cells exposed to some fractions of asphalt fume condensates exhibited significantly reduced DNA content following overnight exposure, but there were no indications of membrane damage or cell killing. The authors conclude that fractionated asphalt fume condensates may have tumor promoting activity by inducing an inhibition of gap junction intercellular communication.
NIOSH-Author; Asphalt-fumes; Carcinogenesis; Carcinogens; Cell-damage; Cell-function; Cytotoxicity; In-vitro-study; Skin-tumors; Toxic-effects; Toxic-vapors