Comparative study of micronucleus assay and chromosomal aberration analysis in V79 cells exposed to ethylene oxide.
Zhong-B; Gu-Z; Whong-W; Wallace-WE; Ong-T
Teratog, Carcinog, Mutagen 1991 Jan; 11(5):227-233
Induction of micronuclei and chromosome aberrations in hamster lung fibroblasts by ethylene-oxide (75218) was examined. Chinese-hamster- V79 lung fibroblasts were cultured and exposed to 3500, 6900, 13,800, or 27,700 parts per million (ppm) ethylene-oxide for 30 minutes. The cells were examined for chromosome aberrations. Other V79 cell cultures were exposed to 457, 1372, 4115, or 12344ppm ethylene-oxide for 30 minutes. The cells were then treated with 3 micrograms per milliliter cytochalasin-B (CytB). The cells were scored for micronuclei. The proportion of binucleated cells was determined. Ethylene-oxide induced significant dose dependent increases in chromosome aberration frequency. The aberrations consisted of chromatid and isochromatid breaks, fragments, minutes, and exchanges. Ethylene-oxide increased the frequency of micronucleated V79 cells; however, only the increase induced by the 12,344ppm exposure was statistically significant. The increases were approximately 1.5 to 3 times higher in cells that had been treated with CytB. The proportion of binucleated cells was increased by CytB but decreased by ethylene-oxide. The decrease induced by ethylene-oxide was dose dependent. The authors conclude that the chromosome aberration and micronuclei induction data indicate that ethylene-oxide is a clastogen. Chromosome aberration analysis appears to be a more sensitive technique for detecting clastogenicity than micronuclei analysis.
NIOSH-Author; In-vitro-studies; Epoxides; Lung-cells; Chromosome-disorders; Dose-response; Clastogens; Cell-division; Mammalian-cells;
Author Keywords: chromosomal aberrations; cytochalasin B; micronucleus formation; chromatid and isochromatid breaks; mononucleated and binucleated cells
Tong-Man Ong, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, 944 Chestnut Ridge Road, Morgantown, WV 26505-2888
Teratogenesis, Carcinogenesis, and Mutagenesis