Use of bacterial assay system for monitoring genotoxic complex mixtures in the occupational setting.
Ong T; Stewart JD; Whong Z
Complex mixtures and cancer risk. Vainio H, Sorsa M, McMichael AJ, eds. Lyon: International Agency for Research on Cancer, IARC Scientific Publications No. 104, 1990 Jan; :101-106
Genotoxicity studies of workplace environments which can be carried out by laboratory analysis or by in-situ assay were discussed. The use of the Ames Salmonella assay system for testing solvent extracts of airborne particle, vapors or gases for mutagenic activity was discussed. Mutagenicity studies were carried out on airborne particles collected from polyurethane manufacturing facilities and from hospital operating rooms where smoke was generated by the use of electrocautery knives or laser beams on human tissues. Drosophila and bacteria have been used for the on site detection of genotoxic agents in the occupational environment. Tests indicate that newer in-situ assays can detect the mutagenic activity of volatile compounds and complex environmental mixtures. This finding supported the suggestion that the index of mutagenicity is a more reliable indicator of health hazards of air pollutants than particulate concentration. The authors conclude that regardless of whether the index of mutagenicity can be used as an indicator of potential health hazards, genotoxicity studies should be performed to generate biological data for the workplace environment whenever possible.
NIOSH-Author; Health-care-personnel; Isocyanates; Toxic-gases; Indoor-air-pollution; Air-quality-monitoring; Epidemiology; Mutagens; Bioassays; Genotoxic-effects; Occupational-exposure; Plastics-industry; Indoor-environmental-quality
Vainio H; Sorsa M; McMichael AJ
Complex mixtures and cancer risk