Induction of sister-chromatid exchanges and chromosome aberrations in human peripheral lymphocytes by 2,4,7-trinitro-9-fluorenone.
Induction of sister chromatid exchanges (SCEs) and chromosome aberrations by 2,4,7-trinitro-9-fluorenone (129793) (TNF) in human lymphocytes was examined. Heparinized peripheral lymphocytes obtained from a healthy male and female donor were cultured and incubated with 0.1, 0.3, 1.0, 3.0, or 10.0 micrograms per milliliter TNF. The cells were assayed for SCEs and chromosome aberrations. TNF induced significant increases in SCE and chromosome aberration frequency in lymphocytes from both subjects. The increases were dose dependent in lymphocytes from one donor. Maximum induction of SCEs amounted to 16.28 and 23.28 SCEs per cell above the control value. Maximum induction of chromosome aberrations in both subjects was four time the control value. Chromatid gaps and breaks were the most frequent type of damage observed. High doses of TNF appeared to be cytotoxic as evidenced by decreases in the number of metaphases and poor chromosome morphology. The authors conclude that TNF is clastogenic and potentially genotoxic. Since TNF has been used in a variety of situations where a potential for occupational exposure exists, the consequences of worker exposure should be a matter of concern.