Potential role of platelet-activating factor in development of occupational lung disease: action as an activator or potentiator of pulmonary phagocytes.
Kang-JH; Dyke-K; Pailes-WH; Castranova-V
This study was designed to investigate whether platelet activating factor may be important in the inflammatory response of the lung to occupational dusts. The in-vitro exposure of alveolar macrophages to cotton dust at 4mg/kg, silica (14808607) at 10mg/ml, or coal dust at 10mg/kg caused a release of picomole levels of platelet activating factor with cotton dust being the most potent and coal dust the least. The platelet activating factor was released from alveolar macrophage about 10 minutes after in-vitro silica exposure. Platelet activating factor is a direct stimulant of polymorphonuclear leukocytes increasing chemiluminescence 2.3 times above control. The response of polymorphonuclear leukocytes to a second stimulant was also potentiated by platelet activating factor. Platelet activating factor was not a direct stimulant of alveolar macrophages in-vitro, having no stimulatory effect on chemiluminescence or superoxide anion release. Platelet activating factor did potentiate zymosan stimulated activation of chemiluminescence and superoxide release from alveolar macrophages by 117 and 30, respectively. The authors conclude that exposure of lung tissue to occupational dusts results in the release of platelet activating factor which may activate polymorphonuclear leukocytes and potentiate the responsiveness of alveolar macrophages to dust.
NIOSH-Author; Respiratory-system-disorders; Lung-cells; Alveolar-cells; Immunology; Blood-analysis; Blood-cells; Dust-exposure; Dust-inhalation; Silica-dusts; Plant-dusts;
NTIS Accession No.
Asthma and Chronic Obstructive Pulmonary Disease; Disease and Injury; Respiratory-system-disorders
National Institute for Occupational Safety and Health