Triphenyl phosphite-induced ultrastructural changes in bovine adrenomedullary chromaffin cells.
Knoth-Anderson-J; Veronesi-B; Jones-K; Lapadula-DM; Abou-Donia-MB
Toxicol Appl Pharmacol 1992 Jan; 112(1):110-119
Transmission electron microscopy (TEM) and scanning electron microscopy (TEM) were used to assess the morphological changes in primary cultures of bovine adrenomedullary chromaffin cells treated with triphenyl-phosphite (101020) (TPP). The cells were isolated and cultured with 100 micromolar (microM) TPP, O,O- diisopropylphosphorofluoridate (55914) (DFP), paraoxon (311455), or vehicle for 24 or 1 hour prior to fixing and TEM examination. For SEM, the cells were similarly treated for 24 hours. Radiolabeled adenosine incorporation into ATP was assessed through 0.25, 0.5, 1, 4, or 24 hour treatments with 100microM of the chemicals and thin layer chromatography. After 24 hours, almost all mitochondria were disrupted or swollen; glycogen buildup was evident. SEM revealed low viability for TPP treated cells. Filopodia were contracted, surface texture was irregular, and giant globular bodies were present. Paraoxon and DFP did not cause the same ultrastructural effects. The authors conclude that TPP's effect is specific and that its target is the mitochondria.
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Organo-phosphorus-compounds; Cell-cultures; Cytotoxic-effects; Nucleosides; Cell-morphology; Microscopic-analysis; Biochemical-tests;
Pharmacology Duke University Department of Pharmacology Durham, N C 27710
101-02-0; 55-91-4; 311-45-5
Neurotoxic Disorders; Neurotoxic-effects
Toxicology and Applied Pharmacology
Duke University, Durham, North Carolina