Depression of contractility in cultured cardiac myocytes from neonatal rat by carbon tetrachloride and 1,1,1-trichloroethane.
Toraason-M; Krueger-JA; Breitenstein-MJ; Swearengin-TF
Toxicol In Vitro 1990 Jul; 4(4-5):363-368
A study was conducted to evaluate the value of using isolated cells for investigating the cardiac effects of halogenated hydrocarbons. Hearts were harvested from Sprague-Dawley-rats. Carbon- tetrachloride (56235) (CCl4) and 1,1,1-trichloroethane (71556) (TCE) had a negative chronotropic effect on myocytes by prolonging the relaxation phase of beating. The duration of the contraction phase of beating, and the peak velocity of cell wall movement were not affected by these halocarbons. By 2.5 millimolar CCl4 or 5 millimolar TCE, beating was completely stopped. Beating activity resumed after these chemicals were washed out. The duration of contraction and relaxation phases of beating were significantly affected by increasing or decreasing the calcium concentration. The changes in calcium concentration did not alter the concentration dependent action of CCl4. The authors conclude that cultured heart cells are useful for assessing the cardiac depressant and sensitizing actions of halogenated hydrocarbons.
NIOSH-Author; Chlorinated-hydrocarbons; Cell-cultures; Cell-function; Cell-damage; Cardiovascular-system-disorders; In-vitro-study; Cardiovascular-function; Halogenated-hydrocarbons
Toxicology in Vitro