Microbial contamination and immunologic reactivity of stored oats.
Olenchock-SA; Sorenson-WG; Marx-JJ Jr.; Parker-JE
Biodeterioration Research 1990 Jan; 3:479-488
An evaluation was conducted to describe the exposure to bacteria, fungi, and endotoxins as well as in-vitro and in-vivo immunologic studies related to the presence of specific antibodies and serum complement activation in farm workers who shoveled approximately 800 bushels of oats from a poorly ventilated storage bin. The evaluation was initiated after a cluster of cases of organic dust toxic syndrome had been reported from this facility. Both the bulk sample and the laboratory generated airborne dust contained gram negative bacterial endotoxins. The contamination of the bulk dust was 122.66 endotoxin units/milligram (EU/mg). The collected dust contained 325.71EU/mg. Calculation of the airborne concentration of endotoxins that was generated in the laboratory resulted in 12865.5 endotoxin units/cubic meter. Most workers reacted positively with Micropolyspora-faeni antigen, but is was not possible with either ELISA or counter immunoelectrophoresis to separate the ill from the workers who were not ill. The extract of bulk oats contained a substance that consumed functional human complement in a dose dependent fashion. The authors conclude that the stored oats provided a source of exposure to microbial antigens and immunoreactive materials.
Immunology; Food-processing-industry; Grain-elevator-workers; Grain-dusts; Mycotoxins; Food-contaminants; Food-handlers; Plant-dusts; Bacterial-dusts; Airborne-dusts; Immunology
Book or book chapter
Biodeterioration Research: Mycotoxins, Biotoxins, Wood Decay, Air Quality, Cultural Properties, General Deterioration and Degradation