A review of safety guidelines and laboratory training related to acquired immunodeficiency syndrome (AIDS) was presented with consideration of recommendations by the Centers for Disease Control (CDC) and the National Institute for Occupational Safety and Health (NIOSH). Discussion of occupational safety guidelines began with explanation of the Health Omnibus Programs Extension Act of 1988 and published responses by NIOSH which were directed at emergency workers, law enforcement workers, and correctional facility personnel. These guidelines offered an overview of modes of transmission, risk assessment, and risk reduction recommendations. The Hospital Infections Program assessed the risk of infection for health care workers, studied exposed workers, and established what are referred to as universal precautions. AIDS programs associated with laboratory training and education were developed by the Public Health Practice Program Office and delivery of the training involved the National Laboratory Training Network and training at CDC headquarters. Discussion of supportive measures for laboratory training focused on the development of training materials and performance evaluations. The authors conclude that several CDC centers offer training and education to health and public safety workers; further, that training efforts are associated with improved diagnoses and clinical management of AIDS cases.