Efficacy of acid/base washes in removing the bioactive agent from cotton dust: response of the guinea pig model to inhalation of treated dust.
Castranova-V; Domelsmith-LN; Barger-MW; Ma-JK; Olenchock-SA; Jones-TA; Frazer-DG
Cotton dust: proceedings of the Fifteenth Cotton Dust Research Conference, beltwide cotton conferences, January 11-12, 1991, San Antonio, Texas. Jacobs RR, Wakelyn PJ, Domelsmith LN, eds. Memphis TN: The National Cotton Council of America, 1991 Jan; :252-255
The efficacy of various cotton fiber treatments in preventing the pulmonary reactions of the guinea-pig model was investigated. The cotton dust was treated with sodium-hydroxide (NaOH) in ethanol overnight at room temperature; with hydrogen-chloride in ethanol for 1 hour at 65 degrees-C, NaOH in ethanol at room temperature for 1.5 hours; or with NaOH in ethanol for 1 hour at 65 degrees, the treatment repeated a total of five times, with the dust being treated with acetic-acid in ethanol for 15 minutes followed by ethanol for 15 minutes at 65 degrees. Guinea-pigs were exposed to 13.0mg/m3 cotton dust for 6 hours and studied over the following 18 hours. The results indicated that the breathing rate, trapped gas, and inflammatory responses of the guinea-pig were not predicted by the measurable endotoxin level of inhaled chemically treated cotton dust. Breathing rate and trapped gas did not correlate with the chemotactic peptide (FMLP) level of inhaled cotton dust. A good correlation was found between neutrophil infiltration, macrophage potentiation, and FMLP content of the dust.
Cotton-dust; Plant-dusts; Bacterial-dusts; Endotoxins; Heat-treatment; Laboratory-animals; Cotton-industry; Pulmonary-function; Byssinosis;
Jacobs-RR; Wakelyn-PJ; Domelsmith-LN
Cotton dust: proceedings of the Fifteenth Cotton Dust Research Conference, beltwide cotton conferences, January 11-12, 1991, San Antonio, Texas