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DNA adducts in humans as biomarkers of exposure to environmental and occupational carcinogens.

Santella RM

J Environ Sci Health Part C Environ Carcinog Ecotoxicol Rev 1991 Jan; 9(1):57-81

https://doi.org/10.1080/10590509109373402

00204078

A review was presented which indicated that the studies on DNA adduct measurement have so far demonstrated that the techniques used have sufficient sensitivity for allowing their application to human samples. The advantages and disadvantages of each method was considered. The immunologic and post labeling methods were not able to provide absolute quantitation of adduct levels. The gas chromatography/mass spectrometry method was highly sensitive and specific, but its cost has precluded its use in large scale epidemiologic studies. While few samples have been analyzed by more than one method, those which have been have frequently presented conflicting results. This conflict was likely related to differences in specificity of the various assays used. Background levels of adducts were observed in most samples including those from unexposed referents. Large interindividual variations in adduct levels have also been observed in individuals with similar exposures. Presumably these differences result from differences in absorption metabolism and repair and demonstrate the advantage of biomonitoring, metabolism and repair over environmental monitoring.

NIOSH-Publication; NIOSH-Grant; Analytical-methods; Chromatographic-analysis; Epidemiology; Chemical-analysis; Analytical-chemistry; DNA-damage; Biological-monitoring

Environmental Sciences Columbia University 650 West 168Th Street New York, NY 10032

JSHREB

19910101

Journal Article

651727.00

Grant

1991

Grant-Number-R01-OH-02622

1

1059-0501

Journal of Environmental Science and Health. Part C: Environmental Carcinogenesis and Ecotoxicology Reviews

NY

Columbia University New York, New York, New York