Surface complexation of Fe(3+) by silica and silicate dusts increases in vitro oxidant generation but diminishes in vitro cytotoxicity.
Division of Allergy, Critical Care, and Respiratory Medicine, Duke University School of Medicine, Durham, North Carolina 1991 Jan; :1-22
A study was conducted to test the hypothesis that in-vitro cytotoxicity by silica (7631869), crocidolite (12001284), kaolinite (1318747), and talc (14807966) correlates with surface complexed iron and subsequent oxidant generation. Silica, crocidolite, kaolinite and talc were either iron loaded in ferric-chloride or wetted with distilled water. The dusts were then examined for in- vitro oxidant generation and cytotoxicity using an assay for lipid peroxidation products of rat liver microsomes and hemolysis, respectively. While thiobarbituric-acid reactive products of microsomes were augmented by surface complexation of iron ions, hemolysis actually decreased. Oxidant generation by these mineral oxides increased over time while membranolysis was immediate and had no correlation with time. The authors conclude that in-vitro oxidant generation by silica and silicate dusts is not associated with hemolysis.
NIOSH-Grant; Pulmonary-system-disorders; Oxidative-processes; Oxidation; Metal-oxides; Cytotoxic-effects; Mineral-dusts
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Division of Allergy, Critical Care, and Respiratory Medicine, Duke University School of Medicine, Durham, North Carolina
University of Tenn Center Health Scien, Memphis, Tennessee