Quantification of volatile solvents in blood by static headspace analysis.
Dills-RL; Kent-SD; Checkoway-H; Kalman-DA
Talanta 1991 Apr; 38(4):365-374
A method was presented for headspace analysis of blood from humans with low level exposure to solvents. Studies performed during the development of the method indicated that the headspace methodology must be optimized specifically for blood, rather than by use of parameters derived from headspace experiments with aqueous media. Procedures used to increase sensitivity in water analyses decreased the sensitivity for blood analyses because of their coagulative effects on blood. For optimum sensitivity and precision, standards and samples must be prepared and manipulated so as to minimize loss of the volatile analytes. Solvents studied in this report included 1,1,1-trichloroethane (71556), toluene (108883), o-xylene (95476), m- xylene (108383), p-xylene (106423), ethylbenzene (100414), styrene (100425), alpha-methylstyrene (98839), and 4-methylstyrene (622979) at concentrations ranging from 0.01 to 1 micrograms/milliliter. The physicochemical properties of the internal standard must be closely matched to those of the analytes for valid quantification with the internal standard method of calibration. The authors were unable to find an internal standard that would be adequate for all compounds investigated.
NIOSH-Publication; NIOSH-Grant; Analytical-methods; Blood-analysis; Analytical-chemistry; Chemical-analysis; Organic-solvents; Solvent-vapors; Biological-monitoring
Environmental Health University of Washington Dept/environmental Hlth/sc-34 Seattle, WA 98195
71-55-6; 108-88-3; 95-47-6; 108-38-3; 106-42-3; 100-41-4; 100-42-5; 98-83-9; 622-97-9
University of Washington, Seattle, Washington