Toxicological effect of vanadium on the macrophage response to Listeria challenge.
Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida 1991 Jun; :1-10
The decreased resistance of mice to Listeria-monocytogenes following vanadium (7440622) treatment was investigated. Female B6C3F1-mice were injected intraperitoneally with ammonium-metavanadate (7803556), every 3 days for 6 weeks, and then injected with 2x10(4) L-monocytogenes. Vanadium exposure interfered with both the uptake and ultimate intraphagolysosomal killing of Listeria. Vanadium treatment resulted in altered patterns of clearance of the bacteria from the peritoneal cavity, liver, and spleen. Peritoneal macrophages from vanadium treated mice were coincubated with Listeria. The total in-vitro phagocytic uptake of Listeria by peritoneal macrophages (PEM) was consistently decreased as a function of infection period. Intracellular killing of Listeria was decreased although PEM from vanadate treated mice were more bacteriostatic than bactericidal. Vanadium exposure resulted in a dose trend depression in three cytoplasmic enzymes crucial to PEM function. Vanadium treatment resulted in a similar decrease in the production of superoxide anion, and an increase in levels of oxidized glutathione. The total glutathione pool was not affected. The authors conclude that the decreased bactericidal activity in PEM of vanadate treated mice was not due to direct inhibition of either activity or synthesis of beta-glucuronidase, N-acetyl-beta-D- glucosaminidase, or lysozyme.
NIOSH-Grant; Grants-other; Enzyme-activity; Laboratory-animals; Vanadium-compounds; Alveolar-cells; Bacterial-infections; Toxic-effects
Food Science & Human Nutrition University of Florida Gainesville, FL 32611 Gainesville, FL 32611
Final Grant Report
NTIS Accession No.
Other Occupational Concerns; Grants-other
Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida
University of Florida, Gainesville, Florida