In vitro glial responses to halothane metabolite, TFA.
Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, Kentucky 1991 May; :1-8
Exponentially grown C6-murine glioma cells were used to study the effects of the halothane (151677) metabolite trifluoroacetic-acid (76051) (TFA) on cell growth; DNA, glycoprotein, and dolichol linked oligosaccharide synthesis; and ribonucleotide triphosphate concentrations. A slight concentration dependent enhancement of cell growth and tritium labeled thymidine incorporation followed 1 day of treatment with TFA. The leucine incorporation was elevated in a time and dose dependent manner following exposure for 1 or 5 days to TFA. The authors suggest that TFA stimulated cell growth and enhanced protein synthesis. A dose dependent effect was also noted on mannose incorporation into glycoprotein and dolichol linked oligosaccharides. TFA accelerated lectin induced cell aggregation. TFA apparently alters plasmalemmal glycoprotein synthesis. According to the authors, these findings should assist in forming a basis for furthering the understanding of the mechanisms underlying halothane associated neurotoxicity.
NIOSH-Grant; Neurotoxic-effects; Protein-chemistry; Nervous-system-disorders; Neurotoxicology; Anesthetics
Anatomy University of Louisville Anatomy and Neurobiology Louisville, KY 40292
Final Grant Report
NTIS Accession No.
Neurotoxic Disorders; Neurotoxic-effects
Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, Kentucky
University of Louisville, Louisville, Kentucky