Effect of quartz dust DQ 12 on human monocytes/macrophages in vitro - an electron microscopical study.
Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 90-108, 1990 Nov; (Part II):1459-1465
An investigation was provided of the surface alterations in human monocytes/macrophages in-vitro induced by quartz (14808607) DQ 12 to explain the considerably higher resistance of human macrophages to quartz particles in-vitro. At concentrations which are highly cytotoxic to guinea-pig macrophages, human monocytes/macrophages reacted with a considerably higher survival rate. Macrophages which had been exposed to 100 micrograms/milliliter of quartz DQ 12 for 48 hours displayed a vacuolar network filled with flocculant material and quartz particles but no signs of cytotoxicity. Results obtained by scanning electron microscopy and by transmission electron microscopy indicated that cultured human monocytes/macrophages displayed unique features on contact with toxic quartz particles. The higher resistance of the cells to quartz concentrations which are toxic for animal cells was underlined by a special mechanism of phagocytosis in combination with autophagolysosomal processes and cell secretion. These effects were species specific characteristics of human cultured monocytes/macrophages which have been exposed to quartz particles.
Lung-cells; Respiratory-system-disorders; Dust-inhalation; Cell-cultures; Dust-exposure; Airborne-dusts; In-vitro-study; Cytotoxic-effects
DHHS (NIOSH) Publication No. 90-108
Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA