Role of mast cells in the pathogenesis of silicosis.
Behrendt H; Ziesche R; Stutz P; Idel H; Friedrichs KH; Hilshcher W; Magnussen H
Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 90-108, 1990 Nov; (Part II):1447-1454
The effects of silica (7631869) dust DQ 12 were investigated on rat mast cells in-vivo and in-vitro. Mast cell topography was also analyzed in transbronchial lung biopsies from three patients with anthracosilicosis. Female Lewis-rats and Wistar-rats were used in these studies. The cytotoxic effect of quartz (14808607) DQ on the rat peritoneal cells was time and dose dependent. Electron microscopic investigation revealed partially degranulated mast cells after incubation with low doses but not with high concentrations of quartz DQ. Early cellular events in rat lungs induced by quartz dust inhalation were characterized by an inflammatory response. Instillation of 40 milligrams quartz DQ 12 into the trachea of rats resulted in lung fibrosis after 8 to 12 weeks. Numerous mast cells were present in transbronchial lung biopsies from silicotic patients. The cells display the species specific whorls and scrolls within their granules. The results indicated that mast cells participate in the generation of silicotic pulmonary fibrosis. Mast cells were clearly involved in both the initial inflammatory process and in fibrogenesis.
Respiratory system disorders; Laboratory animals; Dust inhalation; Silica dusts; Lung disease; Airborne dusts; Lung cells
DHHS (NIOSH) Publication No. 90-108
Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA