In vitro injury to elements of the alveolar septum caused by leukocytes from the bronchoalveolar region of rats exposed to silica.
Donaldson-K; Brown-GM; Slight-J
NIOSH 1990 Sep; :603-606
A study was made of the ability of bronchoalveolar leukocytes from rats exposed to silica (14808607) by a single intratracheal instillation to cause injury to the extracellular matrix and cellular elements of the alveolar septum in-vitro. Leukocyte from rats exposed to two other inflammogenic particulates, a heat killed bacterial preparation, and a yeast cell walls preparation, were similarly assessed for comparison with quartz. Syngeneic PVG-rats, SPF bred, were exposed by intratracheal instillation to 1 milligram of standard quartz, Corynebacterium-parvum, or zymosan. The results indicated that the single injection of silica into the rat lung caused a long term alveolitis. This alveolitis was characterized by a three to 12 fold increase in bronchoalveolar leukocytes comprising 30 to 40% neutrophils. Intratracheal instillation of a heat killed bacterial preparation (C-parvum) or yeast cell walls (zymosan) also caused large scale burst of inflammation immediately following injection, but these resolved quickly returning to near normal levels by 15 days. The authors conclude that the initial severity of the alveolitis is not the main factor in determining the persistence of silicotic inflammation in the intratracheal model.
Mineral-dusts; Airborne-dusts; Fibrous-bodies; Cell-damage; Dust-exposure; Lung-cells; Cell-function; Laboratory-animals; Coal-dust; Respiratory-system-disorders; Silica-dusts; Lung-disease
DHHS (NIOSH) Publication No. 90-108
Proceedings of the VIIth International Pneumoconioses Conference