The dependence of the biological effects in rats on the physical characteristic values of intratracheally tested dusts.
Authors
Rosmanith-J; Schyma-SB; Ehm-W
Source
Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 90-108, 1990 Sep; (Part I):560-565
Abstract
The effect of the physical properties of the coal mine dusts on their specific harmfulness were investigated. Four fractions of different size distributions from four total airborne coal mine dusts sampled on filters were characterized by means of physical methods in their dispersed state. Fifty milligrams of each dust sample were intratracheally instilled in rats as a suspension in 0.5 milliliters of saline solution. The rats were sacrificed 12 months after instillation and the amount of hydroxyproline and lipids as well as the dust mass in the lungs and lymph nodes were determined. The total hydroxyproline correlated with the surface properties of the applied dusts and the total lipids in the lungs with the surface tension of the tested solution. The total lipids were dependent on the hypothetical minimum surface per mass and were reflected by the different surface tension of the tested solution respectively. According to the thermodynamic low the surface tension tended to stop the surface of the phase in minimum. The lipid effect of the tested dusts was produced by their influence on the surfactant system of the lungs and did not reflect the fibrogenicity of the dusts.
Keywords
Mineral-dusts; Airborne-dusts; Fibrous-bodies; Cell-damage; Analytical-methods; Dust-exposure; Silica-dusts; Lung-cells; Cell-function; Laboratory-animals
Document Type
Conference/Symposia Proceedings
Identifying No.
DHHS (NIOSH) Publication No. 90-108
Source Name
Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA
