Sister chromatid exchange frequency and chromosomal aberrations in asbestos factory workers.
Rahman-Q; Nahid-F; Jain-AK; Ayarwal-SS
Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 90-108, 1990 Sep; (Part I):543-546
An investigation was undertaken to evaluate the cytogenetic effects of asbestos (1332214) dust on workers in an asbestos cement factory. Twenty two factory workers and 12 referents were evaluated. From each subject peripheral venous blood was collected. Whole blood lymphocyte cultures was performed. Cultures were harvested at 48 hours to examine chromosomal aberrations and at 72 hours for sister chromatic exchange (SCE) analysis. The aberrant metaphase percentage was significantly higher in exposed smokers and exposed nonsmoker groups in comparison to their respective referent groups. Chromosomal aberrations were of chromatid gap and break type. Mitotic index was low in both groups but not significantly. Mean SCE/cell was elevated significantly without affecting mitotic index and cell cycle kinetics. The authors conclude that the higher SCE/cell in asbestos exposed smokers in comparison with exposed nonsmokers may result from a synergistic action of asbestos and smoking.
Construction-industry; Occupational-exposure; Lung-disease; Pulmonary-disorders; Lung-function; Dust-exposure; Silica-dusts; Lung-cells; Cell-function
DHHS (NIOSH) Publication No. 90-108
Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA