Effect of Metal Elements in Coal Dusts on the Cytotoxicity and Coal Workers' Pneumoconiosis.
Zhang-Q; Yang-S; Wu-X; Tang-S; Xiu-B
NIOSH 1990 Sep:246-249
The effects of nickel (7440020) and zinc (7440666) on the pathogenicity of coal dust were examined. Alveolar macrophages obtained from male Wistar-rats by bronchoalveolar lavage were incubated with samples of dust collected from six Chinese coal mines. The silica (14808607), zinc, and nickel contents of the dusts were determined. The in-vitro cytotoxicity of the dusts correlated well with the incidence of coal workers' pneumoconiosis in the six coal mines. The concentrations of silica in the dusts ranged from 1.55 to 5.45% and did not correlate with cytotoxicity. Nickel and zinc concentrations in the dusts ranged from 38.6 to 155.5 and 73.6 to 342.3 parts per million, respectively. The most cytotoxic dust had the highest nickel concentration. The least cytotoxic dust had the highest zinc concentration. Rat alveolar macrophages were incubated with the most cytotoxic coal dust at concentrations of 0 or 100 micrograms per milliliter (microg/ml) plus 0, 0.2, or 0.4microg/ml zinc-chloride (7646857). The effects on intracellular ATP concentration were determined. Male Wistar- rats were administered 0 or 50mg/ml dust plus 0, 0.1, or 0.2mg/ml zinc-chloride intratracheally. Fifteen days later they were killed and the lungs were removed and lavaged. The alveolar macrophages were recovered and analyzed for potassium (K+) and zinc (Zn+2). In- vitro, coal dust significantly decreased intracellular ATP concentration. Zinc-chloride antagonized the effect. In-vivo, coal dust significantly decreased the macrophage K+ concentration. The decrease was countered by zinc-chloride. Macrophage Zn+2 concentrations were significantly increased in zinc-chloride treated animals. The authors conclude that cytotoxicity of coal dust and the detection rate of CWP are associated with the nickel content of coal dust. Zinc-chloride antagonizes the cytotoxicity of nickel containing coal dust in-vivo and in-vitro.
In-vivo-studies; Laboratory-animals; Coal-dust; In-vitro-studies; Epidemiology; Coal-workers-pneumoconiosis; Metals; Cytotoxic-effects; Alveolar-cells;
7440-02-0; 7440-66-6; 14808-60-7; 7646-85-7;
DHHS (NIOSH) Publication No. 90-108
Proceedings of the VIIth International Pneumoconioses Conference