Inhibition of metabolic coupling by metals.
Loch-Caruso-R; Corcos-IA; Trosko-JE
J Toxicol Environ Health 1991 Jan; 32(1):33-48
This study examined whether certain metal compounds could alter junctional communication. Metabolic coupling was used to assay cell to cell communication in cultured Chinese-hamster-V79 cells. Wild type Chinese-hamster-V79 cells proficient in metabolism of 6- thioguanine (6-TG) were cocultured with mutant V79 cells unable to metabolize 6-TG to its toxic metabolite (resistant cells). Inhibition of metabolic coupling in the presence of 6-TG by the metals was demonstrated as an increase in the recovery of 6-TG resistant cells. Significantly increased recoveries of 6-TG resistant cells were demonstrated by the toxic metal compounds arsenic(V)-acid (1327522), mercurous-chloride (7546307), lead(II)- acetate (15347576), and nickel(II)-chloride (7718549) at concentrations which did not change cell survival. Since the increased recovery with nickel showed no dependence on concentration, its significance may be negligible. Cadmium-chloride (10108642) increased 6-TG resistant cell recovery, but only at a toxic concentration. Zinc-sulfate (7733020) did not increase recovery at all. This cellular response to arsenic (7440382), mercury (7439976), and lead (7439921), the inhibition of junction mediated intercellular communication, has not been previously reported.
NIOSH-Publication; NIOSH-Grant; Reproductive-system-disorders; Cell-cultures; Cytotoxic-effects; Mercury-poisoning; Mercury-compounds; Lead-poisoning; Lead-compounds; Cadmium-compounds; Arsenic-compounds; Arsenic-poisoning; Metal-poisoning
Environmental & Indust Health University of Michigan 109 Observatory Street Ann Arbor, MI 48109-2029
1327-52-2; 7546-30-7; 15347-57-6; 7718-54-9; 10108-64-2; 7733-02-0; 7440-38-2; 7439-97-6; 7439-92-1
Journal of Toxicology and Environmental Health
University of Michigan at Ann Arbor, Ann Arbor, Michigan