Differences in quinone reductase qctivity in primary bone marrow stromal cells derived from C57BL/6 and DBA/2 mice.
Res Commun Chem Pathol Pharmacol 1990 Mar; 67(3):375-386
Differences in quinone-reductase (QR) activity in primary bone marrow stromal cells derived from C57BL/6-mice and DBA/2-mice were assessed. Whole bone marrow and primary cultures of stromal cells cultured from DBA/2-mice had a lower basal level of QR activity compared to those of C57BL/6-mice and exhibited a greater sensitivity to the toxic effects of hydroquinone (123319), (HQ) a metabolite of benzene (71432). There was, however, no difference between the two strains of mice with regard to benzoquinone (583631) or phenol (108952) induced toxicity. Increased QR activity in DBA/2 and C57BL/6 stromal cells could be induced by prior stromal cell treatment with tert-butylhydroquinone (1948330) which resulted in protection against subsequent hydroquinone treatment. The authors conclude that QR activity within the bone marrow may be a determinant of susceptibility of bone marrow derived stromal cells to the toxicity induced by redox active quinone compounds as evidenced by increased sensitivity of DBA/2 bone marrow stromal cells to HQ treatment and the protection against HQ toxicity in the QR induction studies.
NIOSH-Publication; NIOSH-Grant; Laboratory-animals; Biochemical-analysis; Cell-cultures; Bone-marrow; Bone-structure; Toxicology; Toxic-effects; Enzyme-activity; Cell-metabolism
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Research Communications in Chemical Pathology and Pharmacology
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