Macrophage, Particulates, and Carcinogen Metabolism.
Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, Ohio 1988 Sep:16 pages
A study was conducted to determine the relationship of arachidonic- acid metabolism induced by phagocytosis of ferric-oxide (1309371) to altered benzo(a)pyrene (50328) (BaP) metabolism induced by the same particulate. Alveolar macrophages (AM) were obtained by tracheal lavage of 7 to 9 week old male Golden-Syrian-hamsters following anesthetization with phenobarbital. AM labeled with tritiated arachidonic-acid were exposed to ferric-oxide particulate; the total lipids were extracted for determination of radioactivity. The major finding of the study was the absence of arachidonic-acid metabolic capacity in isolated adherent hamster AM. It is not known whether this deficiency is due to an inherent difference in enzymatic capacity or to conditions in the isolation procedure resulting in the destruction of enzymatic capacity. The author concludes that the hamster alveolar macrophage is not an appropriate in-vitro model for this research.
NIOSH-Grant; Pulmonary-system-disorders; Metabolic-study; Laboratory-animals; Lung-cells; Respiratory-system-disorders;
Final Grant Report;
NTIS Accession No.
Asthma and Chronic Obstructive Pulmonary Disease; Disease and Injury; Pulmonary-system-disorders; Respiratory-system-disorders;
Department of Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, Ohio