Glioma proliferation modulated in vitro by isothermal radiofrequency radiation exposure.
Cleary-SF; Liu-M; Merchant-RE
Radiat Res 1990 Jan; 121(1):38-45
The effects of isothermal radiofrequency radiation exposure to human glioma cells were examined. Glioma cells were exposed to continuous wave radiofrequency radiation at 27 or 2450 megahertz (MHz) for 2 hours at 37 degrees-C over the specific absorption range from 0 to 200 watts per kilogram (W/kg). Following the exposures the cells were then cultured at 37 degrees-C. The cellular processes followed to examine the effects of exposure included viability, morphological change, and DNA/RNA synthesis monitored by tritiated uridine and thymidine uptake. Results demonstrated that these 2 hour exposures to either 27 or 2450 MHz radiofrequency radiation in the 5 to 200W/kg range caused dose dependent biphasic changes in the incorporation rates of uridine and thymidine. This response was dependent upon the time following exposure, with incorporation rates being examined 1, 3 and 5 days after exposure. Doses in the specific absorption range less than 50W/kg stimulated DNA/RNA synthesis while higher doses caused decreased precursor incorporation. A kinetic cellular response was suggested by these results since the incorporation of uridine and thymidine was time dependent following the exposure. The authors note that the dose and time dependent responses observed indicate that radiofrequency radiation may influence specific phases of the cell cycle.
NIOSH-Publication; NIOSH-Grant; Cell-biology; Dose-response; Electromagnetic-radiation; Radiation-exposure; Cell-morphology; Cell-cultures; Cellular-function; Cell-metabolism; In-vitro-study
Physiology and Biophysics Medical College of Virginia Box 694, Mcv Station Richmond, VA 23298
Virginia Commonwealth University, Richmond, Virginia