In vitro lymphocyte proliferation induced by radio-frequency electromagnetic radiation under isothermal conditions.
Cleary-SF; Liu-M; Merchant-RE
Bioelectromagnetics 1990 Jan-Mar; 11(1):47-56
Effects of continuous wave (CW) radiofrequency (RF) radiation in human peripheral blood lymphocytes were investigated. Whole human blood was exposed to 27 or 2,450 megahertz (MHz) RF electromagnetic radiation for 2 hours at 37 degrees-C. Mononuclear cells were separated and cultured for 3 days at 37 degrees-C with or without mitogenic stimulation using phytohemagglutinin (PHA). At the end of the culture period lymphocyte proliferation was assayed by 6 hours of pulse labeling with tritium labeled thymidine (3HTdR). A dose dependent, statistically significant increase in the uptake of 3HTdR was noted in PHA activated or unstimulated lymphocytes following exposure to radiation at either frequency at specific absorption rates (SARs) below 50 watts per kilogram (W/kg). Uptake of 3HTdR was suppressed by exposure at 50W/kg or higher, relative to that of control cells. Lymphocyte morphology or viability recorded no detectable effects of RF radiation exposure. The authors conclude, taking into consideration the characteristic temperature dependence of lymphocyte activation in vitro, that the biphasic, dose dependent effects of the radiation on lymphocyte proliferation were not dependent on heating. The authors suggest that RF radiation affects other blood leukocytes such as B-lymphocytes or macrophages. They do not rule out the possibility of direct RF induced immunologic alterations in humans repeatedly exposed to amplitude or pulse modulated RF radiation.
NIOSH-Publication; NIOSH-Grant; Magnetic-fields; Blood-cells; Immune-system-disorders; Immunologic-disorders; Electromagnetic-radiation; Radiation-exposure; Radiation-hazards; Microwave-radiation;
Author Keywords: lymphocyte activation; isothermal control; shortwave; microwave; mitogenic stimulation; biphasic dose dependence
S.F. Cleary, Department of Physiology and Biophysics, Box 551, MCV Station, Virginia Commonwealth University, Richmond, VA, 23298-0551
Virginia Commonwealth University, Richmond, Virginia