Effect of the Cocarcinogen Ferric Oxide on Benzo(a)pyrene Metabolism by Hamster Alveolar Macrophages.
Greife-A; Schoeny-R; Warshawsky-D
Polynuclear Aromatic Hydrocarbons: A Decade of Progress 1988:317-327
The effect of the cocarcinogen ferric-oxide (1309371) (Fe2O3) on benzo(a)pyrene (50328) (BaP) metabolism by hamster alveolar macrophages was assessed. Alveolar macrophages were harvested by tracheal lavage and cultured in siliconized vials. The cultures were divided into six groups and incubated up to 9 hours in the presence of radiolabeled BaP or BaP adsorbed onto the selected dose of Fe2O3. Upon completion of the incubation period, the contents of each vial were centrifuged and assayed for the reduction of cytochrome-C. Addition of Fe2O3 particles resulted in a significant concentration dependent increase in the amount of cytochrome-C reduced in comparison to control; this increase was indicative of phagocytosis. Cytochrome-C reduction reached a plateau at 6 hours, after which time a dose dependent decline in cell viability was observed. The amount of BaP endocytized by cells increased for the first 3 hours and then declined. Total BaP metabolites were greater in cells exposed to Fe2O3 particles than in control cells. The amount of metabolites released into the culture medium increased with the concentration of particles. The authors suggest that more BaP may be endocytized by alveolar macrophages in the presence of particles, or, alternatively, that BaP metabolism may be faster in the presence of particles.
Laboratory-animals; Carcinogens; Pulmonary-function; Lung-cells; Iron-oxides; Alveolar-cells; Cell-metabolism; Phagocytic-activity; In-vitro-studies;
Polynuclear Aromatic Hydrocarbons: A Decade of Progress