Sister-chromatid exchange studies on direct- and indirect-acting clastogens in mouse primary cell cultures.
Soler-Niedziela-L; Ong-T; Krishna-G; Petersen-M; Nath-J
Mutat Res 1989 Dec; 224(4):465-470
Results were presented which represent the induction of sister chromatid exchanges (SCEs) by direct and indirect acting clastogens in a primary mouse bone marrow and spleen cell culture assay. This assay uses a system of metabolic activation in the cell culture procedure to activate indirect acting compounds. Bone marrow and spleen cells from male Crl:CD-1 BR-mice were used in these studies. A direct acting genotoxic agent, 2,4,7-trinitrofluorenone (129793), induced a significant dose related increase in SECs. In both bone marrow and spleen cells a dose of 2.0 micrograms/milliliter caused a three fold increase over the SCE level in control cells. An indirect acting genotoxicant, cyclophosphamide (50180), induced a significant increase in SCEs in the presence of rat liver S9. A dose of 2 micrograms/milliliter resulted in a two fold increase in bone marrow and a greater than five fold increase in spleen cells. The indirect acting genotoxicant benzo(a)pyrene (50328) induced significant dose related SCE responses in both cell types. The authors suggest that primary bone marrow and spleen cell culture systems are able to detect both direct and indirect acting genotoxicants and this feature may be useful for both routine and comparative cytogenetic studies.
NIOSH-Author; Genotoxic-effects; Cell-cultures; Chromosome-damage; Bioassays; Metabolic-study; In-vitro-studies; Laboratory-animals;
Author Keywords: SCE studies; Clastogens; direct- and indirect-acting; Mouse; primary cell cultures; Trinitrofluorenone; Cyclophosphamide; Benzo[a]pyrene
Dr. J. Nath, Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506- 6108 (U.S.A.)
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