Development of a biological chemical test for the potability of water.
Christian-RT; Cody-TE; Clark-CS; Lingg-R; Cleary-EJ
AIChE Symposium Series 1973 Jan; 70(136):15-21
As the future plans for water reuse become more realistic, a test system must be developed which should be relatively simple, reproducible, speedy, and relevant to human health. A biological system that will give a sensitive indication of the presence of agents deleterious to human health by unambiguous changes in biological activity may offer a suitable general test. Mammalian cells in cultures were chosen to be tested as an assay system, as the cell is the smallest self sustaining unit of life and it performs most of the basic functions of whole organisms. Efforts to validate the application of the cell cultures test to the human system in general were described. Studies showing the effects of exposure to coal particles on cell culture systems were reviewed as were studies showing the effects of metals including cadmium (7440439) and zinc (7440666). Similar studies reviewed included the effect of Ohio River water and Reuse Water on the cell culture systems. So far, untreated hospital wastes and water treated only by the reverse osmosis unit of the Medical Unit Self/Contained Transportable system were toxic to the cell culture system. The addition of other treatment steps such as activated carbon and ozonation reduced the cytotoxicity of the water samples tested. The authors conclude that future plans include the testing of the response of the cell culture to known toxic organic chemicals. As this information is gathered, it will be possible to determine what concentrations need to be present before a cytotoxic effect is observed.
NIOSH-Grant; Grants-other; Water-purification; Water-analysis; Cell-culture-techniques; Drinking-water; Mammalian-cells
Environmental Health University of Cincinnati Eden & Bethesda Avenues Cincinnati, Ohio 45219
Other Occupational Concerns; Grants-other
AIChE Symposium Series
University of Cincinnati, Cincinnati, Ohio