Improved methods of producing precipitating Aspergillus antigens.
Ann Allergy 1977 Mar; 38(3):202-205
Improved methods for the production of Aspergillus-fumigatus antigens which are capable of giving sensitive and reproducible results by counterimmunoelectrophoresis (CIEP) were described. The influence of the type of casein hydrolysate used, the influence of the method of incubating the CIEP slide, the effect of freeze pressing and concentrating antigen preparations, the effect age of culture, and the effect of carbon and nitrogen source concentrations on antigen production were examined. Of the three different commercial sources used, polypeptone, neopeptone, and multi-peptone, only the antigen produced with polypeptone formed at least four lines without concentration when tested by CIEP with the reference antiserum. The other two antigens formed no discernible lines on CIEP. With regard to incubation time, several more fine lines were apparent when incubation was extended to 18 hours at 4 degrees-C. Concentrating the clarified extract five fold produced a few more lines but, more importantly, the procedure resulted in stronger lines of precipitation, particularly among the minor antigen antibody reaction lines. Older cultures resulted in preparations yielding fewer lines than younger cultures. The authors conclude that reliable Aspergillus antigens can easily be produced in the clinical laboratory.
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Diagnostic-techniques; Fungi; Antigens
Microbiology West Virginia University Med C Department of Microbiology Morgantown, W VA 26506
Annals of Allergy
West Virginia University, Morgantown, West Virginia