Effects of quinoline and 8-hydroxyquinoline on mouse bone marrow erythrocytes as measured by the micronucleus assay.
Hamoud-MA; Ong-T; Petersen-M; Nath-J
Teratog, Carcinog, Mutagen 1989 Jan; 9(2):111-118
This study was designed to determine the ability of two related compounds, quinoline (91225) and 8-hydroxyquinoline (148243), to induce micronuclei in mouse bone marrow erythrocytes. Six to 8 week old male CD1-mice were treated intraperitoneally with single injections of 25, 50, or 100mg/kg of each chemical. Samples of bone marrow were taken at 24, 48, and 72 hours after injection. A significant dose related increase was noted in the number of micronucleated polychromatic erythrocytes (MPCE) at the 24 hour sampling time for all dose levels. Statistically significant increases were caused in the number of MPCEs by exposure to the 50 and 100mg/kg dose levels at 48 hours. At 24 hours the ratios of polychromatic to normochromatic erythrocytes were lower for treated than control animals. Hydroxyquinoline resulted in some increases in the number of MPCEs over control levels, but this compound induced a significant increase in the number of micronucleated normochromatic erythrocytes (MNCEs) at all three doses levels at 24 hours after treatment. Higher incidence of MNCEs at the 48 and 72 hour points were noted following exposure to both the low and medium dose levels. The authors conclude that the results obtained with HOQ were similar to those from in-vitro genotoxicity assays, that the relationship of HOQ to carcinogenicity remains unclear, and that the toxicity of HOQ is higher than that of quinoline.
NIOSH-Author; Genotoxic-effects; Carcinogens; Blood-analysis; Cell-cultures; Laboratory-animals; Bone-marrow; Cytotoxic-effects; Dose-response;
Author Keywords: genotoxicity; clastogens; carcinogen; short-term assay; industrial chemical
J. Nath, Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506-6108
Teratogenesis, Carcinogenesis, and Mutagenesis