Macrophage regulation of myelopoiesis is altered by exposure to the benzene metabolite hydroquinone.
Thomas-DJ; Reasor-MJ; Wierda-D
Toxicol Appl Pharmacol 1989 Mar; 97(3):440-453
Efforts were made to determine whether decreased support of myelopoiesis by bone marrow stroma after in-vitro hydroquinone (123319) exposure was due to selective toxicity to either bone marrow macrophages or fibroblastoid stromal cells. Mouse bone marrow was used to obtain independent populations of fibroblastoid stromal cells and macrophages which were then exposed in culture to hydroquinone. These cells were reconstituted in culture and assayed for their ability to support granulocyte/macrophage colony formation. Bone marrow derived fibroblastoid stromal cell line identified as LTF and primary fibroblastoid stromal cells derived from bone marrow mixed cultures over a 14 day period were both used in the study. Reconstituted cultures containing hydroquinone treated macrophages supported fewer colonies than did corresponding cultures containing untreated macrophages. Reconstituted cultures containing hydroquinone treated fibroblastoid stromal cells were not affected. Hydroquinone reduced detectable interleukin-1 activity in purified macrophage cultures stimulated with lipopolysaccharide. The findings suggest that bone marrow macrophages, rather than bone marrow fibroblastoid stromal cells, are targets for hydroquinone myelotoxicity. Hydroquinone may selectively interfer with macrophage function, perhaps, by altering macrophage interleukin-1 secretion. The findings suggested that replacement of certain growth factors may be feasible in therapy of patients who present with myelotoxic symptoms caused by exposure to benzene (71432) or other chemical agents.
NIOSH-Publication; NIOSH-Grant; Grants-other; Benzene-poisoning; Metabolites; Myeloid-tissue; Hematopoietic-system-disorders; Carcinogenesis; Cell-cultures
Pharmacology and Toxicology West VA Univ Medical Center Dept of Pharmacology & Toxicol Morgantown, W VA 26506
Other Occupational Concerns; Grants-other
Toxicology and Applied Pharmacology
West Virginia University, Morgantown, West Virginia