Evaluation of Transformation Assay Using C3H 10T 1/2 Cells for Use in Screening Chemicals for Carcinogenic Potential.
Schechtman-LM; Lubet-RA; Kouri-RE; Sivak-A; Tu-AS
NIOSH 1982 Apr:635-670
Various methods used to discover the potential health effects of chemicals and complex mixtures to which individuals can be exposed were reviewed. The mouse-C3H-10T-1/2 cell system was described and its utility as a means of detecting chemical carcinogens was evaluated. The properties of 10T-1/2 cells were discussed, including growth characteristics, in-vivo tumorigenicity of chemically transformed cells, and endogenous hydrocarbon metabolizing capacity. The overall sensitivity and specificity of these cells to various carcinogenic chemicals was investigated. Specific assay requirements were needed to render these cells a generally applicable screening system. Suitable modifications included cell synchronization, variations in target cell density, target cell replating, and the use of exogenous sources of metabolizing activity, for example, primary hepatocytes or S-9. For certain classes of chemical carcinogens, each of these approaches has proven feasible. According to the authors, suitable technical procedures need now to be developed for use as generalized screens for unidentified potential carcinogens.
Screening-methods; Carcinogens; Bioassays; Cell-cultures; Cell-function; Biotransformation; Neoplastic-transformation; Mammalian-cells; In-vitro-studies;
Proceedings of the Second NCI/EPA/NIOSH Collaborative Workshop: Progress on Joint Environmental and Occupational Cancer Studies, September 9-11, 1981, Rockville, Maryland