Changes in in vitro brain and spinal cord protein phosphorylation after a single oral administration of tri-o-cresyl phosphate to hens.
Patton-SE; Lapadula-DM; O'Callaghan-JP; Miller-DB; Abou-Donia-MB
J Neurochem 1985 Nov; 45(5):1567-1577
Endogenous phosphorylation of brain and spinal cord proteins was examined during the development, progression, and improvement in delayed neurotoxicity in adult Leghorn-hens following a single oral exposure to 750mg/kg of tri-o-cresyl-phosphate (78308) (TOCP). At 1, 7, 14, 21, 35, and 55 days after treatment, crude membrane and cytosolic fractions were prepared from the brains and spinal cords of the control and treated hens. TOCP administration conferred calcium and calmodulin dependence on the phosphorylation of a few brain cytosolic proteins and caused an increase in the phosphorylation of a number of other cytosolic and membrane proteins. The effect of TOCP intoxication on in-vitro phosphorylation of brain and spinal cord synaptosomal proteins appeared to follow the time course as described in earlier studies for the morphological appearance of neuronal breakdown products in the nerve endings. It also correlated with the onset of and recovery from the clinical signs of ataxia and paralysis associated with delayed neurotoxicity in the hen. Further studies were being made of the changes in in-vitro brain and spinal cord protein phosphorylation after a single oral administration of tri-o-cresyl phosphate to hens.
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Organo-phosphorus-compounds; Organo-phosphorus-pesticides; Spinal-cord-disorders; Central-nervous-system; Laboratory-animals
Pharmacology Duke University Medical Center Box 3813 Durham, NC 27710
Journal of Neurochemistry
Duke University, Durham, North Carolina