Uroporphyrin I stimulation of collagen biosynthesis in human skin fibroblasts. A unique dark effect of porphyrin.
Varigos-G; Schiltz-JR; Bickers-DR
J Clin Invest 1982 Jan; 69(1):129-135
A study was undertaken to assess the effects of uroporphyrin-I and of light on collagen synthesis in fibroblasts obtained from explant cultures of normal adult human skin. Fibroblast monolayers were preincubated for 24 hours in the dark with or without uroporphyrin-I and then incubated for 24 hours with tritium labeled proline. Accumulation of nondialyzable isotopically labeled hydroxyproline increased 2.7 fold when uroporphyrin-I was included in the incubation medium. This represented a specific increase in collagen synthesis. This increase was time dependent and occurred either in the presence or absence of ultraviolet light. A study was performed to determine whether the increased collagen accumulation in the presence of uroporphyrin-I might be the result of decreased collagen degradation. The study of the effects of uroporphyrin-I and light on the growth of human fibroblasts indicated the growth curves were similar for all cultures including the controls, light without porphyrin, porphyrin without light and porphyrin irradiated daily with 400 nanometer light. There were no obvious differences in cellular morphology in the cultures when examined by phase contrast microscopy. The authors suggest that the sclerodermatous lesions seen in the skin of patients with porphyria cutanea tarda and erythropoietic porphyria may be the result of the novel dark effect of uroporphyrin-I as demonstrated in this study.
NIOSH-Publication; NIOSH-Grant; Dermatitis; Skin-exposure; Ultraviolet-radiation; In-vitro-studies; Metabolic-study; Liver-disorders
Medicine Cleveland V a Hospital 10701 East Blvd Cleveland, Ohio 44106
Journal of Clinical Investigation
Case Western Reserve University, Cleveland, Ohio