Quantification of 5- and 15-HPETE's by direct chemical ionization mass spectrometry.
Gut-J; Jamieson-GC; Trudell-JR
Free Radic Biol Med 1987 Jan; 3(5):323-328
The use of direct chemical ionization (DCI) mass spectrometry (MS) with a polyimide coated probe was found to allow resolution and quantification of 5-hydroperoxyeicosatetraenoic-acid (5-HPETE) and 15-hydroperoxyeicosatetraenoic-acid (15-HPETE) in the intact hydroperoxide and free acid form. DCI/MS was shown to be a useful compliment to other techniques for identifying and quantifying fatty acid hydroperoxides. A unique feature of the method was the use of a polyimide coated fused silica fiber that allows vaporization of the hydroperoxides, with very low excess energy, into the plume of the chemical ionization reagent gas plasma. The technique allows simultaneous detection of 5-HPETE and 15-HPETEs in mixtures and offers good linearity in the 100 nanogram to 100 picogram range. Improvements in the method call for the preparation of oxygen-18 labeled hydroperoxyeicosatetraenoic acids. Addition of a heavy isotope labeled compound to the sample would allow an internal standard method of quantification.
NIOSH-Publication; NIOSH-Grant; Grants-other; Analytical-methods; Chemical-analysis; Analytical-chemistry; Fatty-acids
Anesthesia Stanford University Department of Anesthesia Stanford, Calif 94305
Other Occupational Concerns; Grants-other
Free Radical Biology and Medicine
Stanford University, Stanford, California