Identification of a histamine release inhibitory factor (HRIF) and an inhibitor of histamine releasing factor synthesis (IHS) produced by guinea pig lymphoid cells.
Alam R; Lewis DM; Olenchock SA
Cellular Immunol 1988 Sep; 115(2):447-459
Mechanisms which regulate the production and function of histamine release factor (HRF) in guinea-pigs were investigated. English-short- hair-guinea-pigs were immunized with 0.5 or 25 milligrams of ovalbumin dissolved in PBS and emulsified with either CFA or IFA. One milliliter of this suspension was injected subcutaneously and the dose repeated 2 weeks later. Subsequent stimulation of spleen cells obtained from these animals immunized with high doses of antigen suppressed the spontaneous HRF production. Stimulation with Con-A of spleen cells also inhibited the spontaneous HRF production. A significant inhibition of HRF production was noted when the supernatants from Con-A and antigen stimulated cultures were added to fresh thymocyte cultures. Supernatants inhibited HRF induced histamine release from mast cells. An inhibitor of HRF synthesis (IHS) and a histamine release inhibitory factor (HRIF) were identified by gel chromatography. The results indicated that guinea- pig lymphoid cells produced two inhibitory lymphokines along with HRF upon stimulation with antigens or mitogens. One inhibitor suppresses the production of HRF by lymphocytes and the other inhibits HRF induced histamine release from mast cells. All three lymphokines were detected in most supernatants and the final activity of the supernatant elected the predominant factor present. Even though the relevance of these two inhibitory cytokines in human health and disease are not clear as yet, the authors suggest a role for IHS and HRIF in the efficacy of immunotherapy in some allergic diseases.
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