Evidence that lipid peroxidation in microsomal membranes of epidermis is associated with generation of hydrogen peroxide and singlet oxygen.
Dixit-R; Mukhtar-H; Bickers-DR
Biochem Biophys Res Commun 1982 Mar; 105(2):546-552
Evidence was presented which indicated that hydrogen-peroxide and singlet oxygen may mediate peroxidation of microsomal lipids of the epidermis. Neonatal Sprague-Dawley-rats were sacrificed on day four after birth, and epidermal microsomes were prepared from the skin. Incubation of epidermal microsomes with NADPH or xanthine-oxidase system resulted in significant generation of lipid peroxides as measured by malondialdehyde formation. Epidermal lipid peroxidation was inhibited by catalase and singlet oxygen quenchers, 2,5- dimethylfuran, histidine and beta-carotene whereas hydroxide ion scavengers, benzoate, mannitol and ethyl-alcohol had no effect. No protective effect was noted by superoxide-dismutase on lipid peroxidation. Vitamin-E and cytochrome-C3+ substantially inhibited the epidermal lipid peroxidation. The authors suggest that polyunsaturated lipids of skin microsomes were susceptible to peroxidation. The chemical agents known to protect against the toxic effects of singlet oxygen and hydrogen-peroxide inhibited microsomal lipid peroxidation which suggests that these moieties may be important in mediating the reaction. Enzymic reduction of oxygen in the presence of NADPH in epidermal microsomes resulted in the formation of reactive oxygen species which initiate peroxidative deterioration of membrane lipids in epidermis. This membrane damage can be offset by a number of homeostatic cellular defense mechanisms including that of catalase and singlet oxygen quenchers.
NIOSH-Publication; NIOSH-Grant; Dermatitis; Skin-exposure; Lipids; Lipid-disorders; Tissue-disorders; Microsomal-enzymes; Laboratory-animals; In-vitro-studies
Medicine Cleveland V a Hospital 10701 East Blvd Cleveland, Ohio 44106
Biochemical and Biophysical Research Communications
Case Western Reserve University, Cleveland, Ohio