Hair as a biopsy material. V. Hair metal as an index of hepatic metal in rats: copper and zinc.
Jacob-RA; Klevay-LM; Logan-GM
Am J Clin Nutr 1978 Mar; 31(3):477-480
Hair copper (7440508) and zinc (7440666) concentrations as an index of hepatic copper and zinc content were studied in rats. Male Carworth-rats were raised on diets containing 0 or 14.9 micrograms per gram (microg/g) zinc or 3.2microg/g copper or given drinking solutions containing 0, 10, or 20 microg per milliliter (microg/ml) zinc as zinc-sulfate (7733020), 0, 0.5, or 2.0microg/ml copper as cupric-acetate (142712), or 0 or 5.00microg/ml chromium (7440473) as chromium(III)-acetate (1066304). At 500 days of age they were killed and the livers were removed and fractionated. The whole liver and the nuclear, cytosol, large granule, and microsome fractions were assayed for copper and zinc. The hair was removed and analyzed for copper and zinc. Blood samples which had been obtained 36 days before death were analyzed for cholesterol. The mean hair copper and zinc concentrations of the rats were 9.41 and 198microg/g, respectively. The mean copper concentrations in the liver subfractions were: nuclear fraction 0.78microg/g, large granule fraction 0.51microg/g, microsomes 0.21microg/g, and cytosol 1.61microg/g. The mean plasma cholesterol concentration was 213 milligrams per deciliter. Hair copper concentrations were correlated directly with whole liver, subcellular nuclear and cytosol copper concentrations. Hepatic microsomal copper and plasma cholesterol concentrations were inversely correlated. No such correlations were seen for hair and liver zinc concentrations. The authors conclude that determining hair copper content may be useful for assessing total liver copper concentrations in humans.
NIOSH-Publication; NIOSH-Grant; Grants-other; Trace-metals; In-vivo-studies; Laboratory-animals; Trace-analysis; Biological-material; Biopsy; Serum-cholesterol
Environmental Health Kettering Laboratory Eden & Bethesda Avenues Cincinnati, Ohio 45219
7440-50-8; 7440-66-6; 7733-02-0; 142-71-2; 7440-47-3; 1066-30-4
Other Occupational Concerns; Grants-other
The American Journal of Clinical Nutrition
University of Cincinnati, Cincinnati, Ohio