Maintenance of glutathione levels in renal cortex slices of the rat.
Richardson-RJ; Wilder-AC; Murphy-SD
Proc Soc Exp Biol Med 1977 Jan; 154(3):360-364
The stability of glutathione concentrations in rat kidney cortex slices was examined. Renal cortex slices obtained from male Holtzman-rats were incubated in Krebs-Ringer-bicarbonate (KRB) buffer under 95 percent oxygen and 5 percent carbon-dioxide in the presence or absence of 4 percent bovine serum albumin (BSA) at 37 degrees-C for 30 to 120 minutes. The effects on the glutathione content of the slices were determined. Glutathione concentrations decreased during the incubation period. BSA had no effect on the rate of glutathione disappearance. Rat kidney slices were incubated in KRB buffer containing 0 or 3 millimolar disodium-maleate (371471) for 10 to 120 minutes and the effects on glutathione content were determined. Disodium-maleate had no effect on the disappearance of glutathione between 10 and 60 minutes of incubation; however, for incubation periods of 90 to 120 minutes the rate of decrease was accelerated. Rat kidney slices were incubated with dithiothreitol (27565419), glycine (56406), glutamic-acid (56860), methionine (63683), cysteine (52904), or glutamine (56859) alone or in combination for 60 minutes and the effects on their glutathione content were assessed. Dithiothreitol alone had little or no effect on glutathione. Cysteine alone increased the concentration of glutathione, an effect which was enhanced by dithiothreitol. Methionine alone decreased glutathione content. The other amino acids by themselves had little effect, but when given together they increased the glutathione content. Similar experiments were conducted in which the incubation atmosphere was nitrogen instead of oxygen, the incubation temperature was lowered to 25 and 0 degrees- C, and the oxidative phosphorylation uncoupler dinitrophenol (573568) was present. Conducting the incubation under nitrogen or in the presence of dinitrophenol decreased the glutathione content, whereas lowering the temperature increased glutathione content. The authors conclude that the concentration of glutathione in rat kidney slices depends on overall metabolism and the availability of the component amino acids of glutathione. Enzymatic degradation of glutathione can occur anaerobically, whereas oxidative metabolism is required for glutathione synthesis.
NIOSH-Publication; NIOSH-Grant; Grants-other; Amino-acids; In-vitro-studies; Organo-sulfur-compounds; Chemical-kinetics; Physiological-chemistry; Laboratory-techniques; Biological-material; Biotransformation
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