Detection of DNA aneuploidy by quantitative fluorescence image analysis: potential in screening for occupational bladder cancer.
West-SS; Hemstreet-GP III; Hurst-RE; Bass-RA; Doggett-RS; Schulte-PA
Biological monitoring of exposure to chemicals. Organic compounds. (papers presented at the Symposium on the Biological Monitoring of Exposure to Organic Compounds; St. Louis, Missouri in April 1984 in conjunction with the 187th National Meeting of the American Chemical Society), Ho MH, Dillon HK, eds. New York: Wiley, 1987 Jul; :327-341
Two systems of quantitative fluorescence image analysis for detection of DNA aneuploidy in bladder cells were described. The first system was manual and involved the use of a fluorescence microscope to measure the absolute nuclear fluorescent intensity (ANFI) of acridine-orange (AO) stained cells. The second system was automated and involved the use of the Leitz texture analyzing system (TAS), a computerized quantitative fluorescence image analysis (QFIA) system which automated the ANFI findings. Urine cytology specimens from a symptomatic cancer patient population were used. Of 545 chemical company workers exposed to the known bladder carcinogen 2-naphthylamine (91598), 14 cases of bladder cancer were detected, including three identified by ANFI. ANFI measurements indicative of DNA aneuploidy were found in an additional 22 workers, all of whom had been classified as high exposure. Examination of some of these workers indicated the presence of a damaged urothelium consistent with premalignant conditions. The computerized system performed by the TAS (QFIA) was applied to 96 urine samples from 43 patients with cancer and other urinary problems. Eight of these had tumors already proven by bladder biopsy. The QFIA method detected seven of these tumors based on ANFI findings. The authors suggest that these techniques could be applied to screening asymptomatic workers who have been exposed to chemicals known to induce bladder cancer.
Cytopathology; Urogenital-system-disorders; Microscopic-analysis; Genotoxic-effects; Occupational-exposure; Bladder-cancer; Medical-monitoring
Biological monitoring of exposure to chemicals: organic compounds. (papers presented at the Symposium on the Biological Monitoring of Exposure to Organic Compounds; St. Louis, Missouri in April 1984 in conjunction with the 187th National Meeting of the American Chemical Society)