Determination of acrylamide in nerve tissue homogenates by electron-capture gas chromatography.
Poole-CF; Sye-F; Zlatkis-A; Spencer-PS
J Chromatogr 1981 Nov; 217:239-245
A working procedure was demonstrated for the analysis of plasma spiked with acrylamide (79061) and for the detection of acrylamide in the proximal and distal regions of excised sciatic nerves from rats administered acrylamide subcutaneously. Acrylamide was first converted to its 2,3-dibromopropionamide derivative in aqueous solution, plasma or tissue homogenates by ionic bromination. Subsequent acrylamide determination was made by gas chromatography with electron capture detection using a Traco 560 gas chromatograph with a constant/current pulse/modulated Ni63 electron capture detector and a Hall 700-A electrolytic conductivity detector. The overall recovery at the 10 to 1000 parts per billion level exceeded 80 to 90 percent. The principle loss of acrylamide occurred at the derivatization stage and at the extraction solvent evaporation stage. The limit of detection corresponded to 9.5x10(-12) grams of acrylamide on column or 8.4x10(-9) grams in the final biological extract. The accumulation of free acrylamide in the distal region of the sciatic nerve was studied in male Sprague-Dawley-rats injected daily (5 days per week) with 35mg/kg acrylamide for 0 to 4 weeks. Analysis of nerve tissue showed that accumulation was less than 2 to 8 parts per million. To determine whether pyruvate would inhibit the effect of acrylamide on axonal disintegration, a dietary pyruvate supplement was given to some of the treated animals. No distinction was observed between the accumulation of acrylamide in the nerve fiber of rats receiving the dietary pyruvate supplement and those not receiving the supplement.
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Laboratory-animals; Acrylamides; Chromatographic-analysis; Analytical-methods; Chemical-analysis; Neuropathology; Nerve-tissue
Neuroscience Albert Einstein College 1410 Pelham Parkway Bronx, N Y 10461
Journal of Chromatography
Yeshiva University, New York, New York