Glutathione reductase activity in skin exposed to 4-tertiary butyl catechol.
Yonemoto-K; Gellin-GA; Epstein-WL; Fukuyama-K
Int Arch Occup Environ Health 1983 Dec; 51(4):341-345
Experiments were designed to measure glutathione-reductase (GR) activity in mouse skin treated with 4-tertiary-butyl-catechol (98293) (TBC). Three groups of five Uscd-mice were treated as follows: group 1, left ear with TBC dissolved in dimethyl-sulfoxide (DMSO) and acetone and right ear with DMSO/acetone; group 2, left ear with TBC and right ear no treatment; group 3, left ear with DMSO and right ear no treatment. The TBC solution or the solvent was applied topically three times at 48 hour intervals. TBC treated skin demonstrated significant increases in GR activity. The enzyme levels in the nontreated and solvent treated ears failed to show significant changes. Neither skin color changes nor melanocyte degeneration were observed following TBC treatment. Electron microscopy showed that TBC treatment resulted in pheomelanin formation. The elevation in GR was detectable even before pheomelanin production. This caused the authors to suggest the possibility that occupational leukoderma may be detectable before the clinical change develops and this may help in the health management of this condition.
NIOSH-Publication; NIOSH-Grant; Dermatitis; Skin-exposure; Pigments; In-vivo-studies; Pigmentation-disorders; Enzyme-activity; Laboratory-animals; Diagnostic-techniques;
Author Keywords: Occupational leukoderma; 4-tertiary butyl catechol; Glutathione reductase; Pheomelanogenesis
Dermatology University of California Department of Dermatology San Francisco, Calif 94143
International Archives of Occupational and Environmental Health
University of California San Francisco, San Francisco, California