Analysis of n-hexane, 2-hexanone, 2,5-hexanedione, and related chemicals by capillary gas chromatography and high-performance liquid chromatography.
Nomeir AA; Abou-Donia MB
Anal Biochem 1985 Dec; 151(2):381-388
Analytical methods are described for the separation and quantification of n-hexane (110543) and 11 of its related chemicals in one chromatographic system using two capillary gas chromatography methods. In the first method, the temperature program called for isothermic at 50 degrees-C for 30 minutes, followed by a temperature increase of 10 degrees/minute to a final temperature of 180 degrees- C, which was maintained for 7 minutes. The second method called for the isothermic to be 70 degrees-C for 15 minutes, followed by a temperature increase of 40 degrees/minute to a final temperature of 220 degrees-C. This final temperature was maintained for 5 minutes. With the first method, a linear relationship between peak area and amount injected was observed over a range of 0.2 to 10 micrograms. The minimum detectable amounts were 0.05 microgram for n-hexane, cyclohexane (110827), and 2,5-dimethylfuran (625865) and 0.2 microgram for the other compounds tested. A linear relationship between peak area and amount injected was also observed with the second method. An analysis was also performed using a mobile phase which was a linear gradient of 3 to 55 percent 2-propanol in n- hexane in a period of 1 minute at a solvent delivery rate of 0.8 milliliters/minute. Compounds were detected by monitoring the ultraviolet absorbance of the column eluates and quantitation was achieved by measurement of the peak heights. The method showed linearity over a 100 fold range. The minimum detectable amounts ranged from 2 to 20 micrograms, depending on the compound. A test of these methods was performed by analyzing chicken plasma to which a mixture of chemicals was added. Recovery ranged from 30 to 70 percent.
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Solvents; Chromatographic-analysis; Aromatic-hydrocarbons; Metabolic-study; Hexanes
Dr. M. B. Abou-Donia, Department of Pharmacology, Box 3813, Duke University Medical Center, Durham, N. C. 27710
110-54-3; 110-82-7; 625-86-5
Neurotoxic Disorders; Neurotoxic-effects
Duke University, Durham, North Carolina