Immune responses of cynomolgus monkeys to phthalic anhydride.
Biagini-RE; Bernstein-DI; Gallagher-JS; Moorman-WJ; Knecht-EA; Smallwood-AW; Bernstein-IL
J Allergy Clin Immunol 1988 Jul; 82(1):23-29
The immunologic conditions required for sensitization to phthalic- anhydride (85449) (PA) alone or PA complexed with monkey serum albumin (MSA) as a protein carrier were investigated in cynomolgus- monkeys. Four groups of four animals each were administered ten consecutive weekly subcutaneous injections of 2 milligrams aluminum- hydroxide plus one of the following: 200 micrograms (microg) of PA- MSA; 200microg of PA dissolved in ethanol/saline; 200microg MSA; or ethanol/saline alone. Every 2 weeks during the study, skin testing was performed, and total immunoglobulin-E (IgE) and specific immunoglobulin-G (IgG) and IgE to PA-MSA were determined. The results indicated that PA-MSA injections in monkeys were effective in inducing positive skin sensitivity and statistically significant de-novo synthesis of specific immunoglobulin-G (IgG) to PA-MSA. It appeared that the conditions for maximal production of antibody from PA exposure in monkeys required either prior sensitization to hapten dependent determinants on a carrier protein or prior sensitization of hapten independent new antigenic determinants from exposure to the reactive phthalate group compounds. Workers sensitized to PA occupationally most probably form hapten conjugates with proteins present in the airway or airway epithelium. The lack of cutaneous sensitization to PA or PA-MSA in the group of immunized monkeys exposed to PA alone supports this theory.
NIOSH-Author; Laboratory-animals; Immune-system; Immune-reaction; Pulmonary-system; Skin-tests; Plasticizers
Journal of Allergy and Clinical Immunology