A case of contact dermatitis from exposure to Bacillus-subtilis derived proteolytic enzymes was described. The patient was a 35 year old male mixer for a large bakery who presented with symptoms of palmar dermatitis. The job required the manual addition of sodium-sulfite, phospholux, gelatin, and tablets of concentrated proteolytic enzymes from Bacillus-subtilis to the dough. The dermatitis resolved during a 5 month layoff but recurred upon return to work. Intermittent dermatitis occurred over a 9 month period with severe symptoms after 3 consecutive weeks as a mixer. Biopsy indicated acute spongiotic dermatitis. Acute treatment involved oral prednisone administration and sick leave for 3 weeks. The dermatitis resolved over a period of 6 months following transfer out of the mixing area. Patch tests were negative for Bacillus-subtilis protease, sodium-sulfite, phospholux, and baking gelatin. The authors conclude that the mixer suffered from contact dermatitis attributable to the irritant skin effects of proteolytic enzymes derived from Bacillus-subtilis.