Detection of styrene oxide-DNA adducts in lymphocytes of a worker exposed to styrene.
Liu-SF; Rappaport-SM; Pongracz-K; Bodell-WJ
Methods for Detecting DNA Damaging Agents in Humans: Applications in Cancer Epidemiology and Prevention. Bartsch H, Hemminki K, O'Neill IK, eds., Lyon: International Agency for Research on Cancer, IARC Scientific Publications No. 89, 1988 Jan; :217-222
A study of styrene-oxide (96093) DNA adducts in lymphocytes of a worker exposed to styrene (100425) was conducted. In a preliminary in-vitro experiment, deoxyguanosyl-monophosphate (dGMP) or calf thymus DNA was reacted with styrene-oxide. The reaction mixtures were analyzed for styrene-oxide adducts utilizing a phosphorus-32 (P- 32) postlabeling technique. Five adducts were detected in both the dGMP and DNA preparations and had similar chromatographic properties. The same two adducts accounted for 91 percent of the total in dGMP and 88 percent of the total in DNA. Blood samples were collected from a worker exposed to styrene in a factory where reinforced plastics were manufactured and a worker from the shipping department of the same factory who did not have significant exposure to styrene. The time weighted average exposure of the styrene exposed worker on the day the blood sample was obtained was 96 parts per million. Lymphocytic DNA was isolated from the samples and assayed for styrene-oxide/DNA adducts. In the exposed worker two adducts which corresponded to the two major adducts found in the in- vitro study were detected. No adducts were detected in the unexposed worker. The authors conclude that the observed styrene- oxide/DNA adducts could be responsible for the increases in chromosome aberration frequency seen in workers exposed to styrene. P-32 postlabeling can be used for determining DNA adducts in workers exposed to styrene.
NIOSH-Grant; Grants-other; Blood-cells; Organic-solvents; In-vitro-studies; Occupational-exposure; Nucleic-acids; Chemical-binding; Aromatic-hydrocarbons; DNA-adducts
Biomedical & Environ Hlth Scis University of California School of Public Health Berkeley, CA 94720
Bartsch-H; Hemminki-K; O'Neill-IK
Other Occupational Concerns; Grants-other
Methods for Detecting DNA Damaging Agents in Humans: Applications in Cancer Epidemiology and Prevention, IARC Scientific Publications No. 89
University of California Berkeley, Berkeley, California