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Sister-chromatid exchanges induced by triethylenemelamine: in vivo and in vivo/in vitro studies in mouse and Chinese hamster bone and spleen cells.
Nath J; Krishna G; Petersen M; Ong T
Mutat Res 1988 Sep; 206(1):73-82
The induction of sister chromatid exchanges (SCEs) by triethylenemelamine (51183) (TEM) was studied in mice and hamsters. Male CD1-mice and Chinese-hamsters were injected intraperitoneally with 0 to 405 micrograms per kilogram (microg/kg) TEM. Twenty hours later they were killed and femurs, tibiae and spleens were removed. The marrow was flushed out of the femurs and tibiae. The bone marrow and spleens were analyzed for SCEs. In an in-vivo/in-vitro study, spleen and bone marrow cells from treated animals were cultured for 30 or 40 hours and examined for SCEs. Replicative indices (RIs) based on changes in the first three metaphases were computed from both the in-vivo and in-vivo/in-vitro data. In-vivo, TEM caused dose dependent increases in SCE frequency in bone marrow and spleen cells from both species, the response patterns being similar. In the in-vivo/in-vitro experiment, the frequency of SCEs induced by 405microg/kg TEM was lower than in the in-vivo experiment. Spleen cells from animals exposed to 135 and 405microg/kg TEM yielded a higher SCE frequency than similarly treated bone marrow cells. The RIs showed no significant difference between treated and control animals, except in the in-vivo experiment where the 405microg/kg dose caused a significant cell cycle delay in spleen cells from both species. The authors conclude that TEM can induce SCEs in bone marrow and spleen cells of mice and Chinese-hamsters under both in-vivo and in-vivo/in-vitro conditions.
NIOSH-Author; In-vivo-studies; Genotoxic-effects; Laboratory-animals; In-vitro-studies; Amines; Dose-response; Bone-marrow; Author Keywords: Sister-chromatid exchange; Triethylenemelamine
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