Particle analysis by scanning electron microscopy/energy dispersive x-ray analysis/image analysis.
Stettler-LE; Platek-SF; Groth-DH
Aerosols in the mining and industrial work environments, Vol. 3, instrumentation. Marple VA, Liu BYH, eds. Ann Arbor, MI: Ann Arbor Science, 1983 Jan; 3:1151-1167
Applying an automated scanning electron microscopy/energy dispersive x-ray analysis/image analysis (SEM/EDXRA/IA) technique to analyzing particles extracted from human lung tissue was described. Formalin fixed biopsy samples of lung taken from two males, aged 34 and 59 years, suspected of having occupationally induced lung disease were used. The 59 year old subject had worked as a slagger in a foundry and was diagnosed with silicosis. The 34 year old subject who had worked as a grinder was diagnosed with sarcoidosis. After deparaffinization, the samples were washed overnight in filtered deionized water, freeze dried to constant weight, and ashed in a low temperature asher with an oxygen plasma. The ash from each sample was added to 50 milliliters of a 0.05 percent solution of aerosol-OT in filtered, deionized water. The suspensions were ultrasonicated and filtered. The extracted particles were examined by SEM/EDXRA/IA. Free silica (14808607), aluminum-silicate (14504951), mica like particles, miscellaneous aluminum silicates, and endogenous particles were the most frequently found particles in the tissue from the patient with silicosis. Aluminum (7429905), tungsten (7440337), and free silica were the most frequently found particles in the patient with sarcoidosis. The authors conclude that the particles found in the patients' lungs probably originated in the occupational environment. SEM/EDXRA/IA offers a rapid automated technique for sizing, classifying, and quantitating particles in biological tissue.
Analytical-methods; Microscopic-analysis; Sample-preparation; X-ray-analysis; Trace-analysis; Particulates; Lung-tissue; Pulmonary-system-disorders; Occupational-exposure
14808-60-7; 14504-95-1; 7429-90-5; 7440-33-7
Aerosols in the mining and industrial work environments, Vol. 3, instrumentation